384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. minutes and begin reading microplates with seven mouse clicks. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. x. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. It offers extremely low absorbance background, excellent. 17,000 cells. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. x. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. The luminescence was measured in a SpectraMax. No. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. UV Plate 96 Well, Flat Bottom, Cs 50. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Plus 384. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. White plates reflect the light, enabling even low levels of Luminescence to be detected. No additional manuals are available. Running both standard spectrophotometer and microplate. 001 ABS (displayed), 0. the Molecular Devices SPECTRAmax® 190 and 250. Published Dec 16, 2020 | Updated Dec 16, 2020. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. 00. 300 µL of Detergent Compatible Bradford reagent was added to each well. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. * Note: Programming the washer with a final aspirate height of 4. M2. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 340 PC384. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 5 OD at 492 nm Computer interface USB 2. Cell viability and cytoxicity assays. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. They can be read by most microplate readers. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Next-generation luminescence plate reader. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Contact our support team with your questions regarding Molecular Devices products and applications. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. The 6-well plates were incubated at 37ºC for 9 days before picking. A = 2 - log 10 (%T). Versa Max. Washer maintenance kit: 2 X T 2. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. 96-well plates - Filter plates with 0. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Self-righting base, up to 40 degrees past center without dispenser. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. #E010) Pyroplate 96-well microplate (ACC cat. 1 mL/well of media. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 00 USD. Click SoftMax Pro Software User Guide. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Molecular Devices has provided scientists with . 1. 2 with 1000 cells. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. #CA961) Methods. Or scan up to 384 wells at any time to determine the best assay conditions. explore more. Artel MVS Bundle DM Std/800TSNB Reader Details. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Selects either 96-well or 384-well plate for a stand-alone read. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. Microplate Spectrophotometers. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Absorbance cuvette port. We would like to show you a description here but the site won’t allow us. Fluorescence Microplate Reader Comparison Chart. Compare. Learn how you can utilize microplate readers to drive landmark discoveries. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Materials. From the dropdown Menu, select Wellplates. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. 5 fg per well to 0. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. (Molecular Devices cat. VIS absorbance detection. • Photometric range: 0. HeLa. 003 fmol 0. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. No tools required to assemble. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Compare specifications and request a quote from leading suppliers. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. FlexIII. We would like to show you a description here but the site won’t allow us. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. Infinite possibilities. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Our. x. The opaque walls prevent stray light from being detected from neighbouring. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Incubation. 2. Quantitative, whole well cell confluence for every well. ~ Bio-Tek ELx808 Microplate Reader $2,795. Click Create. Lucid Scientific. with Quartz ABS Plastic. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Tristar 3 Multimode Microplate Reader. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Related products. 100 μL of working reagent was added to each well after the 10-minute preincubation step. 000 – 4. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. These robust readers have been placed in labs from Antarctica to. 1. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 5032338. Four wash/rinse bottles are included and configurable for both 96- and 384. M5. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. Bowman Dispensers. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Place the microplate in the drawer. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Wavelength(s):. 2. x. Primary considerations when choosing a microplate reader are throughput and flexibility. Materials. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Detection of KASP genotyping reactions. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. 96 well plate injection time with SpectraMax® L plate reader. Dual injectors for kinetic assays. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. 3% in white 96- and 384. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. 2 OD at 200 nm; this decreased to 0. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Highly selective for dsDNA over ssDNA or RNA. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. , Corning, NY) or Greiner. $488. 2. T = I/I 0 and %T = 100 (T). This page contains the. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The CellTiter-Glo assay was used to measure ATP. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. 0 . Top or bottom detection is available with a simple click of a button. Return to top. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The device was able to achieve 3σ limits of detection of ca. Optional 16-way manifold. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. as well as all other Molecular Devices' microplate readers. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. Compact designs make these instruments. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Contents of wells were pipetted up and down several times to mix. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Browse for different fluorescent readers below and compare across different instrument suppliers. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Capabilities. 2. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. 0 . 1 fmol luciferase per well. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 00. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. Corning. Supersedes 1. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. the Gemini XPS reader. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 100 fmol 1536-well (8 µL) 0. Multimodal plate reader: Yes: 25 mm: Yes—Max. TNF standard curve. Features. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 0 . After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Populate the following info under Description. The plate reader optics are tunable for. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. 00. Select models. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Select up to 3 products. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Values are averages from 5 clean plates, each filled with 200 µL water/well. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 2% in. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. x. 1. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. White 96-well plate Plate formats. It also integrates with the. 315 can't find zero order. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. x. May require LS-50B…. 5in (46. 00. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. 1. #CA961) Methods. Or scan up to 384 wells at any time to determine the best assay conditions. 020 fmol 0. Microplates, cuvettes (via adapter) Detectors. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Mode Plate Reader. B. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. 3. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. We would like to show you a description here but the site won’t allow us. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Another consideration, says Amouretti, is plate format. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Growth medium was then replaced with fatty acid uptake solution. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. 384-WELL SBS STERILE MICROPLATE. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Place the microplate in the drawer. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Gemini XPS Reader scans. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Range of 96, 384-well SBS microplates . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. The plate reader can measure multiple readouts, including UV and. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. maximum-illumination-channels. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. Learn more. Absorbance Linearity: 2. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Description. The Versamax Microplate Reader is built around a grating monochromator. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. tools to expand the boundaries of their research. Fluorescence Microplate Reader Comparison Chart. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. 1% in white 96- and < 0. Automated live cell imaging – cell counting and confluence. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. A = Log 10 (I 0 /I). Validation made easy -1) Select the correct plate and the wells that you want to measure. Page 1 of 8 . Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. Silica excitation fiber, glass emission fiber. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Controlled by the computer. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. Microplate readers, SpectraMax®, with SpectraDrop. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. EMax. Reader Documentation Version No. x. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. 5 ng per well. a white 96-well half-area microplate (Corning cat. . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. UV Plate 96 Well, Flat Bottom, Cs 50. Compare Tool. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 25% and 0. com. Microplate Reader. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Top and bottom reads are. QC tools and IQOQ services. 0025 OD from 0. Pick the best speed, sensitivity, and price for your needs. REF (SpectraMax. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Specifications subject to change without notice. 012 fmol 0. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Mix samples in microplate wells by user-programmable Automix feature. Sort By: Show:. 76358-624. Loading Buffer was also added to a set of cell-free wells for background controls. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. In addition, the iD5 reader measures TRF and FP and. Polypropylene deep well plates, round bottom. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. On the day of the assay, the plate was equilibrated. 9000-0756. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The IC50s by cell rounding and LysoTracker. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. Luminescence applications and assays. x. 046 to 0. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. . Site. Important things to consider when purchasing a multimode microplate reader. to each well. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. Turn the instrument around so that the back of the instrument is. $3,450. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. High-throughput, automated dynamic light scattering. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range.